Varotari was born in Padua on 4 April 1588 and was initially trained, according to the sources, by his father Dario, a well-known architect and painter from Verona. This seems highly unlikely, as Dario died around 1596–97, when Alessandro was still a child. Recent studies suggest that his artistic education unquestionably took place within the sphere of the family’s friends and acquaintances. In any case, he moved to Venice in 1614 and was a registered member of the painters’ guild there from 1615 to 1639. He made a trip to Rome for educational purposed some time after 1614 and came into contact there with the artistic innovations of the Carracci brothers. The numerous dated works still in existence make it possible to trace the course of the artist’s career and constant fidelity to the style of Palma the Younger and Titian with a certain degree of consistency. His last surviving documented work is the Soldiers’ Altarpiece or Pala delle Milizie (1641) in the Cathedral of Palmanova. He died on 20 July 1649.